Sample Quality and Submission Conditions for NGS

Sample is vital to mitigate any degradation and give your samples the best possible chance of passing sample and library QC. The following steps will ensure that your samples are in the best possible state after shipping.

DNA Quality:

·         Double-strand, non-degraded, and containing no particulate matter

·         Purified by column or gel purification protocols

·         A260/280 ratio of 1.8 or above

RNA Quality:

·         RNA integrity Number value should be greater than 8

·         28S rRNA band at 4.9kb should be twice the integrity of the 18S rRNA band at 1.9kb

Sample Quantity:

·         DNA sample should be measured by “Picogreen method”.

·         RNA sample should be measured by Ribogreen or 2100 bioanalyzer RNA chip.

DNA Submission Condition

·         Submit sample DNA in TE buffer at a minimum concentration of 100ng/㎕ in a clearly labelled 1.5~2.0ml microcentrifuge tube sealed with parafilm tightly.

·         Include a brief description of the DNA extraction and/or enrichment protocol used in the “Comments” fields of the sample order sheet.

·         Certain carriers and pellet paint types are not compatible with aspects of our sequencing technology.

·         Non-robust results have been observed from samples where Lithium Chloride (LiCl), fluorescent pellet paint, or tRNA was used prior to precipitation and resuspension for sample preparation input.

RNA Submission Condition

The best condition for RNA storage or delivery is Ethanol precipitation condition (stable for 1 year at -20C); All solutions should be used with DEPC-treated water.

1. Add 0.1 volume of 3 M Sodium Acetate (NaAce, pH7-8) to RNA solution and mix gently.

2. Add 2 volume of 100% ethanol to RNA solution and mix gently.

ex) If you have 100㎕ of RNA solution, add 10㎕ of 3 M Sodium Acetate (NaAcetate, pH7-8) and 220㎕ of 100% ethanol to RNA solution and mix well gently.

3. Send with Blue Ice Packs.

·         Submit sample RNA in a clearly labeled 1.5~2.0ml microcentrifuge tube sealed with parafilm tightly.

·         Include a brief description of the RNA extraction and/or enrichment protocol used in the “Comments” fields of the order sheet.

Certain carriers and pellet paint types are not compatible with aspects of our sequencing technology. Non-robust results have been observed from samples where Lithium Chloride (LiCl), fluorescent pellet paint, or tRNA was used prior to precipitation and resuspension for sample preparation input.